punctaspecks graphical user interface (gui) (MathWorks Inc)
Structured Review

Punctaspecks Graphical User Interface (Gui), supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
Images
1) Product Images from "PunctaSpecks: A Tool for Automated Detection, Tracking, and Analysis of Multiple Types of Fluorescently Labeled Biomolecules"
Article Title: PunctaSpecks: A Tool for Automated Detection, Tracking, and Analysis of Multiple Types of Fluorescently Labeled Biomolecules
Journal: Cell calcium
doi: 10.1016/j.ceca.2020.102224
Figure Legend Snippet: Various steps used by PunctaSpecks for noise removal and puncta identification. (A) Synthetic movie stack containing dynamical activity of objects under investigation (only 5 frames are shown). (B) Maximum intensity profile (MIP) image of the whole movie. (C) Puncta identified by PunctaSpecks using a given algorithm (Otsu in this case) are encircled on the MIP image to ease the visualization and further adjustment of parameters for accurate detection of all puncta. Histograms of the areas (D), mean fluorescence intensity (E) of all puncta, and (F) mean square displacement as a function of time for the mobile puncta.
Techniques Used: Activity Assay, Fluorescence
Figure Legend Snippet: Verifying the performance of PunctaSpecks using synthetic data. (A) Frame-by-frame signal to noise ratio of the synthetic data at two different noise levels. Histograms of areas (B), mean open times (log scale) (C), and dwell times (log scale) (D) of all puncta with true (blue) and those from PunctaSpecks at noise levels 25 (green) and 150 (red). (E) Histogram of mean intensities of all puncta with true values (blue) and those from PunctaSpecks at noise level of 25 (green) and 150 (red). The actual trajectory of a sample mobile punctum (blue) and the one identified by PunctaSpecks at noise level of 150 (red) (F).
Techniques Used: